细胞DNA定量分析技术相关文献

作者：吴燕萍, 马文霞

关键字：宫颈上皮内瘤变，液基细胞学

摘要：

目的：分析对比应用细胞电脑扫描计算机辅助细胞学检测系统（CCT）、液基细胞学检查计算机辅助细胞学检测系统（LCT）及DNA定量细胞学检测3种方法对诊断宫颈上皮内瘤变（CIN）的意义。[方法]分别采用CCT、LCT及DNA定量细胞学检测，对宫颈细胞学异常者或有临床异常者，行阴道镜下活检。对比3种细胞学检测方法的CIN检出率。[结果]CCT和LCT检测与阴道镜下活检病理结果符合率对比，差异有显著性。LCT和DNA定量细胞学检测与阴道镜下活检病理结果符合率对比，差异无显著性。[结论]LCT和DNA定量细胞学检测由于采用液基薄层制片，较CCT常规宫颈涂片，明显降低假阴性率，并提高了对低度鳞状上皮以上病变的诊断率。DNA定量细胞学可测定DNA含量的改变及检出DNA倍体异常细胞。定期检测可对病变的性质及发展趋势作出客观估计和预测。

作者：Martial Guillaud, Calum MacAulay

关键字：宫颈癌，液基细胞学，HPV

摘要：

Abstract Because 80% of cervical cancers arise in low-resource settings, many inexpensive strategies are being tested. In that spirit, the authors are testing large-scale genomic or DNA ploidy measurements as an inexpensive and semiautomated strategy. Patients entered either a screening or diagnostic study of several optical technologies: quantitative cytology, quantitative histopathology, and fluorescence and reflectance spectroscopy using a point probe, a multispectral digital colposcope, or a combination of the two. We calculated sensitivities, specificities, positive and negative predictive values, and their confidence interval testing conventional cytology, Hybrid Capture (HC) II testing, and DNA ploidy measured on the Feulgen-stained quantitative Pap smear. The current investigation reports on 1555 patients for whom colposcopically directed biopsies were read 3 times by study pathologists. The final histopathologic diagnosis was high grade (cervical intraepithelial neoplasia [CIN] 2, CIN 3, carcinoma in situ [CIS], and cancer) in 16% of patients. Using high-grade squamous intraepithelial lesions (SILs) histopathology as the threshold and gold standard, the sensitivity and specificity, respectively, were: 0.47 and 0.96 for conventional cytology, 0.91 and 0.80 for HC II, and 0.59 and 0.93 for DNA ploidy. The positive and negative predictive values (PPV, NPV) for conventional cytology were 0.70 and 0.90, 0.46 and 0.98 for HC II, and 0.63 and 0.92 for DNA ploidy. DNA ploidy shows comparable sensitivity, specificity, PPV, and NPV values to conventional cytology and HC II. Unlike conventional cytology, DNA ploidy is semiautomated and can be performed in less than 8 hours. Cost effectiveness studies are under way, but in the authors' laboratory DNA ploidy is inexpensive.

作者：夏潮涌, 孙小蓉

关键字：宫颈癌

摘要：

篇首： 贵刊2005年第34卷第7期435页刊载的"DNA倍体分析系统用于宫颈癌及上皮内瘤变的诊断及预测"一文,有几个问题尚持有不同看法,愿意提出来与作者商榷.