细胞DNA定量分析技术相关文献

作者：樊精湛,

关键字：液基细胞学，宫颈病变

摘要：

目的:通过对宫颈细胞 TBS+DNA倍体定量检测,及早发现宫颈病变,阻断宫颈癌的发生。方法:对妇科门诊就诊的1000例妇女进行宫颈细胞TBS+DNA倍体定量检测,阳性 患者再做宫颈活检。诊断标准采用TBS分类系统。结果:1000例妇女宫颈刮片标本,TBS+DNA倍体定量检测阳性53例,阳性率为5.3%,其中不典 型腺细胞(AGCUS)2例,低度鳞状上皮内病变(LSIL)40例,高度鳞状上皮内病变(HSIL)9例,鳞状细胞癌(SCC)2例。53例阳性者进行 宫颈活检,其中轻度不典型腺上皮增生2例,CINⅠ39例,CINⅡ7例,CINⅢ3例,SCC2例。宫颈活检对TBS+DNA倍体检测提供的阳性病变加 以确诊。30～40岁年龄阶段是CIN的高发年龄,发病率为56.6%(30/53)。结论:宫颈细胞TBS+DNA倍体定量测定与病理组织学有一定的相 关性。TBS+DNA倍体定量测定作为无创伤检测是门诊筛查子宫颈癌的有效方法。对年轻妇女应特别关注宫颈癌的门诊筛查工作。

作者：黄卫彤, 韦莹慧

关键字：不能明确意义的非典型鳞状上皮细胞，宫颈病变

摘要：

 宫颈不典型鳞状上皮细胞（atypical squamous ceell，ASC），是指形态改变较良性反应性改变明显，但在数量和程度上又不足以诊断为鳞状上皮内病变的一组阴道、子宫颈的细胞病理改变。由于ASC病理改变多样，临床转归差异显著，对于ASC病例的诊断及处理，有的学者主张采用细胞学随访观察，

作者：黄宏思, 黄燕

关键字：宫颈癌筛查

摘要：

目的 比较传统细胞学与细胞DNA倍体分析系统对宫颈病变筛查敏感性,寻找早期筛查宫颈癌和癌前病变敏感的方法.方法 将1 206例妇科检查的对象随机分为传统组争DNA倍体分析组,分别采用传统宫颈细胞学与细胞DNA倍体分析系统进行宫颈早期病变筛查,对筛查阳性病例进行阴 道镜下活检,并对两组阳性结果进行比较分析.结果 检测显示DNA倍体分析组筛查阳性率(4.47%)和宫颈上皮内瘤样变(CIN)检出率(82.22%)均高于对照组(1.32%、25.00%),两者 比较差异有统计学意义(P＜0.01).结论 细胞DNA倍体分析检测方法对宫颈病变筛查阳性率和CIN检出率高于传统宫颈细胞学检查,是早期筛查宫颈癌和癌前病变敏感而准确的方法.

作者：焦红丽, 夏潮涌

关键字：宫颈癌筛查，HPV

摘要：

在宫颈癌筛查中,联合应用DNA倍体分析和高危型人乳头状瘤病毒（HR-HPV）检测,为临床宫颈癌疑似病例、病理诊断有疑义病例的鉴别诊断提供一种有效的方法。方法：对239例细胞学筛查有异常的病例进行病理组织学检查,随后再次液基薄层细胞学制片、Feulgen染色,做细胞核DNA倍体分析。对有HPV感染的病例,将其检查结果与DNA倍体分析结果进行比较。结果：239例患者中114例进行了HPV检测,其中HR-HPV感染病例55例,出现异倍体69例,二者阳性检出率均与宫颈病变程度相关;HR-HPV感染病例中发现大部分有异倍体产生,二者均不受年龄影响。结论：HR-HPV感染可能导致DNA异倍体的出现,细胞核DNA倍体分析和HPV检查结果又均与病变的程度密切相关,二者联合可为疾病的筛查、预后的判断提供更多有价值的资料。

作者：黄卫彤, 曲德英

关键字：液基细胞学，宫颈病变

摘要：

宫颈癌是最常见的女性生殖道恶性肿瘤,近年来根据临床资料显示,老年妇女宫颈癌高发年龄在55～70岁,而且发病的临床特点是发现病变较晚,病情较严重,死亡率高.因此,老年妇女应重视宫颈癌筛查.本文对1 210例妇女进行宫颈液基细胞DNA倍体检测结果分析.

作者：David C. Wilbur, Wolfgang Klietmann

关键字：应用研究

摘要：

CONTEXT: -Whole-slide imaging technology offers promise for rapid, Internet-based telepathology consultations between institutions. Before implementation, technical issues, pathologist adaptability, and morphologic pitfalls must be well characterized. OBJECTIVE: -To determine whether interpretation of whole-slide images differed from glass-slide interpretation in difficult surgical pathology cases. DESIGN: -Diagnostically challenging pathology slides from a variety of anatomic sites from an outside laboratory were scanned into whole digital format. Digital and glass slides were independently diagnosed by 2 subspecialty pathologists. 

Reference, digital, and glass-slide interpretations were compared. Operator comments on technical issues were gathered. RESULTS: -Fifty-three case pairs were analyzed. There was agreement among digital, glass, and reference diagnoses in 45 cases (85%) and between digital and glass diagnoses in 48 (91%) cases. There were 5 digital cases (9%) discordant with both reference and glass diagnoses. Further review of each of these cases indicated an incorrect digital whole-slide interpretation. Neoplastic cases showed better correlation (93%) than did cases of nonneoplastic disease (88%). Comments on discordant cases related to digital whole  technology focused on issues such as fine resolution and navigating ability at high magnification. CONCLUSIONS: -Overall concordance between digital whole-slide and standard glass-slide interpretations was good at 91%. Adjustments in technology, case selection, and technology familiarization should improve performance, making digital whole-slide review feasible for broader telepathology subspecialty consultation applications.